FAQ

Nov yog cov kauj ruam dav dav rau kev siv lub tshuab centrifuge:

1. Kawm kom paub txog lub tshuab centrifuge uas koj yuav siv. Nyeem cov lus qhia ntawm lub chaw tsim khoom thiab paub txog cov khoom sib txawv thiab qhov chaw teeb tsa.

2. Xyuas kom tseeb tias lub tshuab centrifuge ua haujlwm zoo thiab kho kom raug rau daim ntawv thov tshwj xeeb uas koj yuav siv nws.

3. Muab cov qauv kuaj tso rau hauv lub centrifuge rotor. Cov qauv kuaj yuav tsum tau faib kom sib npaug thiab sib npaug hauv lub rotor kom tsis txhob muaj kev puas tsuaj rau lub tshuab.

4. Kaw lub hau ntawm lub tshuab centrifuge kom ruaj khov. Lub tshuab yuav tsis ua haujlwm tshwj tsis yog tias lub hau kaw kom raug.

5. Teem qhov ceev thiab lub sijhawm uas koj xav tau rau lub tshuab centrifuge. Cov chaw teeb tsa tshwj xeeb yuav nyob ntawm hom qauv uas tab tom ua thiab cov txheej txheem uas koj ua raws.

6. Pib lub tshuab centrifuge. Nco ntsoov sawv deb ntawm lub tshuab thaum lub sijhawm ua haujlwm kom tsis txhob raug mob los ntawm cov khoom txav mus los.

7. Thaum lub tshuab centrifuge khiav tiav lawm, ua tib zoo tshem cov qauv ntawm lub rotor. Nco ntsoov ua tib zoo tuav cov qauv thiab tsis txhob cuam tshuam cov txheej uas yuav tau tsim thaum lub sijhawm centrifuge.

8. Pov tseg cov khoom pov tseg raws li cov txheej txheem kev nyab xeeb thiab ntxuav lub tshuab centrifuge kom huv si ua ntej thiab tom qab siv.

Nws yog ib qho tseem ceeb uas yuav tsum nco ntsoov tias cov tshuab centrifuge tuaj yeem ua rau muaj kev phom sij yog tias tsis siv kom raug. Ib txwm ua raws li cov lus qhia kev nyab xeeb thiab tau txais kev cob qhia kom raug ua ntej siv lub tshuab centrifuge.

Kev siv centrifugation yog ib txoj kev siv ntau los cais cov khoom sib txawv ntawm cov ntshav hauv lub hnab ntshav.

Nov yog cov kauj ruam dav dav uas koom nrog hauv kev centrifuging lub hnab ntshav:

1. Xyuas kom tseeb tias lub hnab ntshav muaj cov ntaub ntawv ntawm tus neeg mob thiab hom ntshav uas nyob hauv.

2. Muab lub hnab ntshav tso rau hauv lub tshuab centrifuge. Lub tshuab yuav tsum tau teeb tsa raws li cov lus qhia ntawm lub chaw tsim khoom thiab calibrate rau hom ntshav tshwj xeeb uas tab tom ua.

3. Kaw lub hau ntawm lub tshuab centrifuge thiab pib txoj kev tig. Qhov ceev thiab lub sijhawm ntawm kev tig yuav nyob ntawm hom ntshav uas tab tom ua.

4. Thaum lub hnab ntshav tig, cov khoom sib txawv ntawm cov ntshav yuav sib cais raws li lawv qhov ceev. Cov qe ntshav liab hnyav dua yuav nyob hauv qab ntawm lub hnab, thaum cov plasma sib dua yuav nce mus rau saum.

5. Thaum cov txheej txheem tig tiav lawm, ua tib zoo tshem lub hnab ntshav tawm ntawm lub tshuab centrifuge. Nco ntsoov tuav lub hnab maj mam kom tsis txhob cuam tshuam cov khoom sib cais.

6. Tam sim no cov khoom sib cais tuaj yeem rho tawm ntawm lub hnab ntshav siv cov khoom siv hloov ntshav uas tsis muaj kab mob. Cov qe ntshav liab tuaj yeem siv rau kev hloov ntshav, thaum cov plasma tuaj yeem siv rau lwm yam kev kho mob.

Nws yog ib qho tseem ceeb uas yuav tsum nco ntsoov tias yuav tsum tau kev cob qhia thiab kev ceev faj kom zoo thaum kov cov ntshav thiab siv cov tshuab centrifuge.

Qhov ceev uas yuav tsum tau siv los cais cov ntshav siv lub centrifuge nyob ntawm hom ntshav uas koj xav cais thiab hom centrifuge uas siv. Feem ntau, rau cov hom phiaj kho mob niaj hnub, qhov ceev centrifugation feem ntau siv los cais cov ntshav yog ntawm 3000 txog 4000 revolutions ib feeb (RPM). Qhov ceev no txaus los cais cov ntshav ua nws cov khoom, uas yog cov qe ntshav liab, cov qe ntshav dawb, thiab cov plasma.

Txawm li cas los xij, yog tias koj xav tau kev sib cais cov khoom ntawm cov ntshav tshwj xeeb dua, xws li kev cais qee hom cell lossis cov khoom me me, koj yuav tsum tau kho qhov ceev ntawm kev centrifugation raws li qhov ntawd. Piv txwv li, txhawm rau cais cov platelets ntawm plasma, qhov ceev ntawm kev centrifugation tuaj yeem nce mus txog li 4000-5000 RPM.

Nws yog ib qho tseem ceeb uas yuav tsum nco ntsoov tias qhov ceev ntawm centrifugation yuav txawv nyob ntawm hom centrifuge, hom raj siv, thiab qhov ntim ntawm cov qauv uas raug centrifuged.

Yog li ntawd, nws raug pom zoo kom ua raws li cov lus qhia tshwj xeeb rau koj lub centrifuge thiab hom raj tshwj xeeb kom ua tiav cov txiaj ntsig zoo tshaj plaws.

Kev sib npaug ntawm lub centrifuge yog qhov tseem ceeb kom ntseeg tau tias cov qauv tau faib sib npaug thaum lub sijhawm tig, uas tuaj yeem pab tiv thaiv kev puas tsuaj rau lub centrifuge thiab txhim kho qhov tseeb thiab kev rov ua dua ntawm cov txiaj ntsig.

Nov yog cov kauj ruam dav dav los sib npaug lub centrifuge:

1. Xyuas qhov siab tshaj plaws ntawm lub centrifuge thiab tsis txhob tshaj nws.

2. Muab cov raj lossis cov chaw tuav cov qauv tso rau hauv lub centrifuge rotor raws li tus qauv sib npaug ncig lub axis nruab nrab ntawm lub rotor.

3. Yog tias tsim nyog, ntxiv cov raj khoob lossis cov hnyav sib npaug rau cov chaw khoob hauv lub rotor kom ua tiav qhov sib npaug.

4. Kaw lub hau centrifuge thiab xyuas kom meej tias nws tau ruaj khov.

5. Teem lub centrifuge mus rau qhov ceev uas xav tau thiab khiav nws rau lub sijhawm luv luv.

6. Nres lub centrifuge thiab xyuas seb puas muaj kev co lossis kev co. Yog tias lub centrifuge tsis sib npaug, nres lub centrifuge, qhib lub hau, thiab rov kho cov khoom tuav qauv lossis ntxiv lossis tshem tawm cov hnyav sib npaug raws li qhov xav tau.

7. Rov ua cov kauj ruam 5 thiab 6 kom txog thaum lub centrifuge khiav zoo yam tsis muaj kev co lossis kev co.

Nws yog ib qho tseem ceeb uas yuav tsum ua raws li cov lus qhia tshwj xeeb rau koj lub centrifuge thiab hom rotor kom ntseeg tau tias muaj kev sib npaug zoo. Tsis tas li ntawd, kev saib xyuas thiab kev kho kom raug ntawm lub centrifuge tuaj yeem pab kom ntseeg tau tias muaj cov txiaj ntsig zoo sib xws thiab raug.

1. Kev Ceev: Tus neeg siv khoom xav siv thiab hom qauv uas lawv yuav ua haujlwm nrog yuav qhia qhov ceev ntawm lub centrifuge. Cov rotors sib txawv haum rau qhov ceev sib txawv, yog li nws tseem ceeb heev uas yuav tsum nug tus neeg siv khoom tias lawv xav tau qhov ceev siab npaum li cas thaum cais cov qauv.

2. Peev Xwm thiab ntau npaum li cas: Yuav tsum xav txog qhov loj me ntawm cov qauv thiab qhov yuav tsum tau ua ntawm cov neeg siv khoom. Muaj pes tsawg milliliters ntawm cov qauv (lub raj centrifuge) uas tus neeg siv khoom xav tau cais, thiab ntau npaum li cas txhua cov qauv yuav tsum tau cais ib zaug yog cov yam tseem ceeb hauv kev txiav txim siab txog lub centrifuge tsim nyog.

3. Kev tswj qhov kub thiab txias: Qee cov qauv kuaj xav tau kev tswj qhov kub thiab txias tshwj xeeb thaum lub sijhawm centrifugation, yog li nws yog ib qho tseem ceeb uas yuav tsum xav txog seb lub centrifuge puas tswj tau qhov kub thiab txias lossis tsis. Yog tias tus neeg siv khoom xav tau cais cov qauv kuaj ntawm qhov kub thiab txias uas tau teev tseg, lawv yuav tsum xaiv lub centrifuge uas tswj tau qhov kub thiab txias.

4. Lub kaw lus tswj: Yuav tsum xav txog lub kaw lus tswj ntawm lub centrifuge, tshwj xeeb tshaj yog tias tus neeg siv khoom xav tau cov haujlwm tshwj xeeb xws li kev txheeb xyuas rotor, xauv kev nyab xeeb, lossis qhia txog qhov txhaum.

5. Qib suab nrov: Yog tias lub centrifuge yuav siv rau hauv chav kuaj mob nrog lwm yam khoom siv lossis cov neeg ua haujlwm, nws yog ib qho tseem ceeb uas yuav tsum xav txog qib suab nrov ntawm lub centrifuge thiab xaiv tus qauv suab nrov tsawg dua kom tswj tau qhov chaw ua haujlwm zoo.

6. Cov Khoom Siv Ntxiv: Qee qhov kev sim xav tau cov raj centrifuge tshwj xeeb lossis cov thawv kuaj, yog li nws tseem ceeb heev uas yuav tsum xav txog qhov muaj cov khoom siv ntxiv thaum pom zoo rau lub centrifuge rau cov neeg siv khoom.

Los ntawm kev xav txog cov yam no thiab nkag siab txog cov kev xav tau tshwj xeeb ntawm cov neeg siv khoom, koj tuaj yeem muab cov lus qhia zoo rau lub centrifuge zoo rau lawv lub chaw kuaj mob.

Qee cov khoom siv tsim nyog rau lub centrifuge suav nrog:

1. Cov rotors: Lub rotor yog ib feem ntawm lub centrifuge uas tuav cov raj lossis cov thawv uas tab tom tig. Nws yog ib qho tseem ceeb uas yuav tsum xaiv lub rotor uas sib xws nrog cov raj lossis cov thawv uas koj yuav siv.

2. Cov Adapters: Cov adapters yog qhov tsim nyog rau kev siv cov raj lossis cov thawv ntawm ntau qhov ntau thiab tsawg dua li lub rotor tsim rau.

3. Cov raj centrifuge: Muaj ntau hom raj centrifuge sib txawv rau ntau hom qauv, xws li cov raj conical hauv qab, cov raj puag ncig hauv qab, lossis cov raj tiaj hauv qab. Nws yog ib qho tseem ceeb uas yuav tsum xaiv hom raj uas tsim nyog rau koj daim ntawv thov tshwj xeeb.

4. Cov tes tsho raj: Cov tes tsho raj yog qhov tsim nyog thaum centrifuging qee hom qauv, xws li cov qauv tshuaj lom lossis cov qauv radioactive, los muab ib txheej ntxiv ntawm kev nyab xeeb.

5. Cov hnab ntshav: Cov hnab ntshav yog cov thawv tshwj xeeb uas siv rau kev centrifugation ntawm cov qauv ntshav.

6. Cov khoom siv tswj qhov kub thiab txias: Qee cov centrifuges muaj peev xwm tswj qhov kub thiab txias, thiab cov khoom siv xws li cov rotors tswj qhov kub thiab txias lossis cov blocks qauv yuav tsim nyog rau qee qhov kev siv.

7. Cov khoom siv roj-nruj: Cov khoom siv no yog qhov tsim nyog thaum ua haujlwm nrog cov qauv uas tsis muaj zog, vim lawv tiv thaiv kev ua kom qhuav lossis kev ua qias tuaj ntawm cov qauv thaum lub sijhawm centrifugation.

Thaum xaiv cov khoom siv rau koj lub centrifuge, nco ntsoov xaiv cov uas sib xws nrog koj tus qauv tshwj xeeb ntawm centrifuge thiab koj cov kev xav tau sim.

Qhov chaw tsawg kawg nkaus uas xav tau los khaws lub kiv cua rau sab nraub qaum ntawm lub centrifuge yuav nyob ntawm qhov loj thiab tsim ntawm lub kiv cua. Feem ntau, yuav tsum muaj qee qhov chaw seem nyob ib puag ncig lub kiv cua kom muaj cua nkag tau zoo, thiab tiv thaiv nws ntawm kev puas tsuaj.

Kev co hnyav tuaj yeem tshwm sim thaum lub rotor thiab phab ntsa sab hauv ntawm lub tais centrifuge tsis sib dhos zoo, thaum lub rotor raug overloaded, lossis thaum lub rotor sib npaug tsis ua haujlwm. Txhawm rau tiv thaiv qhov no, tus neeg siv yuav tsum xyuas kom meej tias lub rotor thiab lub tais tau sib dhos zoo, tias lub rotor tsis raug overloaded, thiab tias lub rotor tau sib npaug zoo.

Qhov sib txawv tseem ceeb ntawm cov rotors ceev siab thiab qis ntawm cov centrifuges yog lawv qhov ceev tshaj plaws. Cov rotors ceev siab tuaj yeem ncav cuag qhov ceev txog li 100,000 revolutions ib feeb (rpm), thaum cov rotors ceev qis feem ntau siab tshaj plaws ntawm kwv yees li 10,000 rpm. Qhov ceev tshaj plaws uas lub rotor kab rov tav feem ntau tuaj yeem tiv taus nyob ntawm ntau yam, xws li nws qhov loj me, cov khoom siv, thiab kev tsim. Txawm li cas los xij, feem ntau cov rotors kab rov tav tuaj yeem tiv taus qhov ceev txog li 30,000 rpm yam tsis muaj kev puas tsuaj loj lossis kev ua tsis tiav. Nws yog ib qho tseem ceeb kom ua raws li cov lus qhia ntawm cov chaw tsim khoom thiab cov lus qhia kev nyab xeeb thaum siv lub centrifuge kom tsis txhob raug mob lossis puas tsuaj rau cov khoom siv.

Lub centrifuge rotor yog qhov tig ntawm lub centrifuge qhov twg cov qauv raug tso rau kev sib cais.

Muaj ntau hom rotors sib txawv rau cov ntawv thov tshwj xeeb, tab sis cov khoom ntawm ib qho rotor thiab cov haujlwm uas lawv ua yog:

1. Lub cev: Lub cev ntawm lub rotor yog lub qauv tseem ceeb uas txuas rau lub cev muaj zog ntawm lub centrifuge thiab tuav lwm qhov chaw rau hauv qhov chaw.

2. Thoob: Txhua lub thoob yog ib lub thawv me me uas tuav cov qauv uas yuav tsum tau cais tawm. Cov thoob muaj ntau qhov ntau thiab tsawg thiab cov ntaub ntawv nyob ntawm seb daim ntawv thov tshwj xeeb yog dab tsi.

3. Lub hau: Lub hau ntawm lub rotor yog qhov uas kaw lub thoob kom cov qauv nyob hauv qhov chaw thaum lub sijhawm centrifugation.

4. Adapter: Ib qho adapter yog ib qho khoom siv uas haum rau hauv lub thoob thiab ua rau lub rotor tuaj yeem haum rau cov raj ntawm ntau qhov ntau thiab tsawg lossis duab.

5. O-ring: O-ring yog ib lub gasket uas tsim ib lub foob ntawm lub hau thiab lub thoob. Nws tiv thaiv kev xau thiab kev ua qias tuaj ntawm cov qauv thaum lub sijhawm centrifugation.

6. Cov ncoo raj: Cov ncoo raj raug muab tso rau hauv lub thoob lossis lub adapter los ua kom cov qauv ruaj khov thiab tiv thaiv nws kom tsis txhob puas thaum lub sijhawm centrifugation.

7. Spindle: Lub spindle yog qhov nruab nrab ntawm lub rotor uas tuav txhua yam ua ke thiab sib txuas ntawm lub rotor thiab lub cev muaj zog centrifuge.

Zuag qhia tag nrho, cov no yog cov khoom tseem ceeb ntawm lub centrifuge rotor. Txawm li cas los xij, cov khoom thiab lawv cov haujlwm yuav txawv nyob ntawm hom thiab daim ntawv thov ntawm lub centrifuge, thiab qee lub rotors yuav muaj cov khoom ntxiv xws li tes tsho lossis nplhaib kom haum rau cov qauv loj dua.

Lub centrifuge yog ib lub cuab yeej uas siv lub zog centrifugal los cais cov khoom sib txawv hauv cov kua lossis sib tov.

Nws muaj ntau qhov tseem ceeb, txhua tus ua haujlwm tshwj xeeb:

1. Lub cev muaj zog: Qhov no yog qhov uas muab lub zog rau lub centrifuge, muab lub zog tig uas tsim nyog.

2. Rotor: Qhov no yog ib feem ntawm lub centrifuge uas tuav cov qauv uas yuav tsum tau cais tawm. Nws tig nrog lub cev muaj zog, tsim kom muaj kev nrawm ntawm centripetal uas xav tau rau cov txheej txheem cais tawm.

3. Cov Tuav Raj: Cov no yog cov qhov uas cov raj kuaj tau muab tso rau hauv lub rotor, tus lej thiab qhov loj ntawm cov raj no sib txawv ntawm lub centrifuge mus rau lub centrifuge nyob ntawm lawv cov lus qhia.

4. Lub hau: Lub hau tsim ib lub foob airtight hla lub rotor thiab tiv thaiv cov ntsiab lus los ntawm ya tawm thaum lub centrifuge ua haujlwm.

5. Vaj Huam Sib Luag Tswj: Qhov no yog ib feem ntawm lub centrifuge uas cia tus neeg siv saib xyuas thiab kho qhov chaw. Nws muaj ntau lub khawm thiab lub pob qhov rooj los tswj qhov ceev, lub sijhawm, thiab qhov kub ntawm centrifugation.

6. Nres: Thaum lub sijhawm xav tau rau kev centrifugation tas lawm, nres yuav ua rau lub rotor qeeb ntawm qhov ceev tshaj plaws uas nws tau mus txog. Zuag qhia tag nrho, kev tsim kho thiab tsim qauv ntawm lub centrifuge tuaj yeem sib txawv raws li daim ntawv thov tshwj xeeb, tab sis cov saum toj no yog cov khoom tseem ceeb ntawm lub centrifuge ib txwm muaj.

Nws yog ib qho tseem ceeb kom txheeb xyuas qhov ua rau muaj suab nrov thiab daws qhov teeb meem sai sai kom tiv thaiv kev puas tsuaj rau lub centrifuge lossis nws cov ntsiab lus.

Tej zaum yuav muaj ntau yam ua rau lub centrifuge nrov. Qee qhov ua rau muaj xws li:

1. Tsis sib npaug: Yog tias qhov thauj khoom hauv lub centrifuge tsis faib sib npaug, nws tuaj yeem ua rau lub centrifuge co thiab tsim suab nrov.

2. Cov kabmob uas hnav lawm: Raws li lub sijhawm dhau mus, cov kabmob hauv lub centrifuge tuaj yeem hnav tawm thiab ua rau lub rotor co, ua rau muaj suab nrov.

3. Cov khoom xoob: Cov khoom xoob lossis puas lawm, xws li siv, ntsia hlau, lossis ntsia hlau, tuaj yeem ua rau lub centrifuge co thiab tsim suab nrov.

4. Kev Thauj Khoom Ntau Dhau: Kev khiav lub centrifuge nrog qhov hnyav ntau dhau tuaj yeem ua rau nws ntxhov siab thiab ua suab nrov.

5. Siv Tsis Yog: Yog tias lub centrifuge tsis siv raws li cov lus qhia ntawm cov chaw tsim khoom, nws tuaj yeem ua rau muaj suab nrov.

6. Laus lawm: Cov qauv centrifuges qub dua yuav tsim suab nrov dua vim hnav thiab tsim kua muag dhau sijhawm.